LOCUS       pMD8_dpy-10_sgRNA       3456 bp ds-DNA     circular     09-FEB-2016
DEFINITION  [Boulin Lab] sgRNA expression vector targeting the d10 site.
KEYWORDS    "sgRNA"                                                            
COMMENT     [Boulin Lab, 2017] sgRNA expression vector targeting the d10 site.                                                  
FEATURES             Location/Qualifiers
     primer_bind     complement(1245..1268)
                     /label="pJET_rev AAGAACATCGATTTTCCATGGCAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        372..871
                     /label="U6 pol III promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    872..891
                     /label="CRpMD8"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     305..324
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     310..332
                     /label="pJET_fwd CGACTCACTATAGGGAGAGCGGC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    892..974
                     /label="sgRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2407..3066)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1627..2309)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tCTCCAAGAA CTCGTACAAA AATGCTCTGA AGTAGGTCTC GAGATCAATA
      421 CTGGGAAGAC GAAAGTCTTG CGAAACCGAT TCGCTGACCC CAGTAAAGTC TACTTCGGTA
      481 GCCCTTCCCC CACCACCCAG CTCGACGACG TCGACGAGTA CATCTACCTC GGTCGTCAAA
      541 TCAACGCCCA AAACAACTTG ATGCCGGAAA TCCACCGAAG ACGTCGAGCA GCCTGGGCTG
      601 CATTCAATGG AATCAAGAAT GCCACCGACT CCATCACCGA CAAGAAGATT CGTGCGAATC
      661 TGTTCGACTC AATTGTCCTT CCAGCGCTCA CCTACGTTTC AGAAGCCTGG ACATTCACCA
      721 AAGCTCTATC CGAACGAGTA CGAATCACAC ATGCCTCCCC CACAAATTAT TGATCTACAT
      781 CCCCATTTGG TATAGTGTCT TACAGGACTC TCAATATGTC AGCTGCATGA TAAATGGTCC
      841 CTATATAATT GAATTGCAAA TCTAAATGTT TgCTACCATA GGCACCACGA GGTTTTAGAG
      901 CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG
      961 TCGGTGCTTT TTTTCAATAA TATTCTAGAT ATCCCTTTTT GAAACTAAAA TGAAATACTC
     1021 TAAATTTTTG AGTTCAATGT TTCAACTAAT CTTGGTTAAA GGTAAATTCC CGGAGGGACG
     1081 ATTTTAAAGT TGCCAGAGTT GATTAAATTG GTCCAAAAAA TTTTATTAGG AAACTCGAAA
     1141 AAAAATTGAT TTTTTTTGAC AATATTCCAA AGAAAATTAC AAAATCTCGA CGCCAAACAT
     1201 AGTCGGCTGT Gatctttcta gaagatctcc tacaatattc tcagctgcca tggaaaatcg
     1261 atgttcttct tttattctct caagattttc aggctgtata ttaaaactta tattaagaac
     1321 tatgctaacc acctcatcag gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact
     1381 ctcatgaaaa ctacgagcta aatattcaat atgttcctct tgaccaactt tattctgcat
     1441 tttttttgaa cgaggtttag agcaagctgc ttcctcgctc actgactcgc tgcgctcggt
     1501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2701 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3001 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3361 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     3421 gacattaacc tataaaaata ggcgtatcac gaggcc
//