LOCUS       pSEM89_egl-23::SL2::wrmScarlet 6880 bp ds-DNA     circular     20-FEB-2017
DEFINITION  .
COMMENT     [Boulin Lab, 2017] contains wrmScarlet cDNA sequence (no introns) fused downstream of gpd-2/gpd-3 SL2 transplicing sequence. 
FEATURES             Location/Qualifiers
     misc_feature    390..583
                     /label="exon 10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2438..3133
                     /label="wrmScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2185..2187
                     /label="PAM"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(6596..6614)
                     /label="oBP225"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2192..2194
                     /label="STOP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     146..165
                     /label="T7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1857..2191
                     /label="exon 13"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    complement(5197..191)
                     /label="pUC19 backbone"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(6023..6682)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(5243..5925)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2430..2437
                     /label="Linker"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    958..1128
                     /label="exon 11"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2165..2184
                     /label="CRpCL11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    192..2191
                     /label="LHR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3137..5196
                     /label="RHR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2195..2429
                     /label="gpd-2/gpd-3 transplicing seq"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1534..1677
                     /label="exon 12"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3134..3136
                     /label="ajout STOP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacaaacttg gtacctaata cgactcacta tagggGGCGC GCCtcgaCCT
      181 GCAGGtcgaG CTGGCTATAT GGTTTGGAGG AAAGAAgtaa gttctcagtc tcaatagatt
      241 ttagttatac aactttattt tcaaagaagc tctgaaacgg agaatttttt tcaaaaacaa
      301 aaattgttct actgtttcaa cagaactatt taataacaac tatctgaact gtagtattca
      361 caatggcatg gtggtattaa ttttttcagA ATTACAATGA AAGCACTGGT CAAAAACCTC
      421 GGTGACCAGT TCAATCTTCC AACTACCGTA GTCAAGAATT TGAATTTGGA TCATTTTGTG
      481 GATCAAGCGA TTAAAGTGGA GGAAGGAGAG ATTGAGACAC TCAGACCGCC GCCTTATGAG
      541 CCACCGTCTG ATCGATTTGA AGCTGAATTC GCTGATGAAC CAGgttagtt gaaactgaaa
      601 actgtaagga agttgtttgt atcgaaactg ttatataaaa attgctgatt tttttttaaa
      661 tttttacctt tttcaagctg aaatttaaaa aatcaaaaca attcaaaaaa aaatctgata
      721 aaatttgact ttgaatattg gttttccgat tttccatata tcaaaaatgt aaaaaaataa
      781 taataaacta ttaatgttct ttgtagaggt agttaaaaat aatattccac tgaaaaaagc
      841 tatttttcaa ttcaaatatt tattaatgtt tcacaattaa attatatact gaaacagccc
      901 acgtttgaag ttatctttta tttgtgtgat tctaatgaat atgcagtgca aattcagAAT
      961 GTGAATGGAT CCGTGATCCG ACTCCAACTC CACCCCCATC ACCTCAACCG GTTTATCGTC
     1021 TTCCATCTCC AAAACCAGTT ACACCGGAAC CTCTACCAAG TCCAACAATA ACTGATGTAT
     1081 CACTTGCGAT TGCAACACCA TCACCTGAAG AATCGGATGA TGATCAAGgt ttgatgttag
     1141 agagtgaaca tattttagtt tgattttttg atagaaattc ggttaatgat tgattttttt
     1201 ttgaaaaaaa ttatgtttca aactatttat ttgaaccagg gctttttaac cgaatattgc
     1261 tgaagatact tggttcttat acattactac attacgagct gaggctcgct caaataaatt
     1321 aaaaatccta atagaagcac tctgatatca catctcgctc aaaaaacatc gagattattt
     1381 tgagaaaaca attacagttt caaggaacca agaaaacgcc ttaaaagttt tcctgtttaa
     1441 ttttactaac cgctcaatat ctaggcatga aattcagtta taaccttggc tgaaaccttg
     1501 aaaatctaga aattacctat cacatctttc cagAACTAAT TCTCCCATCA CCTGAACCGA
     1561 GTCCAGTTCG AGAGCCAACT CCACCACCGC CTCCACGGGA GCCAACACCT CGTGAGCCAA
     1621 CTCCTGAGCC AGAGCCAGTT CGAGAGCCGA CGCCTCCACC TCCGCCACCT GCCAAGCgta
     1681 agtttatatt aacaagatag ctatgtttgt tgtatgaaat agtaaaagtt tcgccgattt
     1741 tttacaaaaa ttccttgagt ttaacatttt actgtttcta cagtttgata caatttgatg
     1801 cagtttataa tatttcaatc tatggttcag tcaatcttac gaatttttat ttccagCCCG
     1861 TCCACTGACT GCCGCTGAAA TCGCGGCTCA AAAACGCAAA GCGTACAGCG AAGAAGCATG
     1921 GCGTCGATAC CAAGAATACC AGAAACAATG GAAGAAGTTC CGTCAAACTC AGAAAACTCC
     1981 CGCACCATCT GGAGCCTCTA CATCAGGGGC ATCAACATCA AAACCGTCTG GAACATCACC
     2041 GGAAAGTGGA GCCGGAGTAT GTACTGGACC ATCAACAAGG AGTCAATCAA TAACATCAGT
     2101 TGCATCTGGA AAGACATCAA GAAGTGCAAC ACCGGAAAGC AAGAAATCAT CACATTTGAG
     2161 TGGCTCATCG AGAAGAGAAA GCGGTGGAAA ATAAgctgtc tcatcctact ttcacctagt
     2221 taactgcttg tcttaaaatc tatgcttctc tttagtatct aaaattttcc tagaagctta
     2281 caagtatata aatggtctct tctcaataaa ggttgtatat ttattcatct tattgaatct
     2341 gccatttcct cgtttttgcg agtttatata ccttccaatt ttctttctat tgtattttca
     2401 acttctaatt ttaattcagg gaaactgctG GCCGGCCATG GTCAGCAAGG GAGAGGCAGT
     2461 TATCAAGGAG TTCATGCGTT TCAAGGTCCA CATGGAGGGA TCCATGAACG GACACGAGTT
     2521 CGAGATCGAG GGAGAGGGAG AGGGACGTCC ATACGAGGGA ACCCAAACCG CCAAGCTCAA
     2581 GGTCACCAAG GGAGGACCAC TCCCATTCTC CTGGGACATC CTCTCCCCAC AATTCATGTA
     2641 CGGATCCCGT GCCTTCACCA AGCACCCAGC CGACATCCCA GACTACTACA AGCAATCCTT
     2701 CCCAGAGGGA TTCAAGTGGG AGCGTGTCAT GAACTTCGAG GACGGAGGAG CCGTCACCGT
     2761 CACCCAAGAC ACCTCCCTCG AGGACGGAAC CCTCATCTAC AAGGTCAAGC TCCGTGGAAC
     2821 CAACTTCCCA CCAGACGGAC CAGTCATGCA AAAGAAGACC ATGGGATGGG AGGCCTCCAC
     2881 CGAGCGTCTC TACCCAGAGG ACGGAGTCCT CAAGGGAGAC ATCAAGATGG CCCTCCGTCT
     2941 CAAGGACGGA GGACGTTACC TCGCCGACTT CAAGACCACC TACAAGGCCA AGAAGCCAGT
     3001 CCAAATGCCA GGAGCCTACA ACGTCGACCG TAAGCTCGAC ATCACCTCCC ACAACGAGGA
     3061 CTACACCGTC GTCGAGCAAT ACGAGCGTTC CGAGGGACGT CACTCCACCG GAGGAATGGA
     3121 CGAGCTCTAC AAGTAAttaa aacttgtgta atttttaatg aaaataaagt tttgtgctac
     3181 gaatttacat ttttcagtgt ttctagcagc ggacactcgc agacttgaat aaaattaaac
     3241 tcgtaaaaaa agaatattcg attcattgaa tcaatgaaac caatttatta atttatttgt
     3301 tttaagaaat cgatcacaaa tatgcttgga aattgcagta tcttttcaaa ttttcaaacg
     3361 aatctggcat ctcccaaaat ccaactctat tgtagacaaa aaggtctatt acttattaaa
     3421 ttactatttc ttattaaaac tattacttat taaattgata taaattaaaa tattctaaaa
     3481 gaatccttta tttatcaggt tgtcccataa gtttttgtac tatttttttt ttgaaaaaaa
     3541 tttatttctc tcaagcgaca agtagtacta ttcacacaag tattcaccat tagtgtccac
     3601 cacttgttgc catttactag gtaacatcat gatgccacgg gagaagaaat ccggcgaacg
     3661 cgatgagaag aaagtggaga gttcagtttt gagatgctct tcgtcgtcga attgcttgtc
     3721 gcgcatgtag tcactgagag acaagaacaa atggtagtcg gttggtgcaa gatctggaga
     3781 atatggtgga tgcggtaaaa cagtccaacc aagatcttgc agcttttgga aagtcttctt
     3841 ggcgacatga ggcctagcat tatcgtgaag aaaatatagt ttttcatatt ttccgttggt
     3901 cttttctgca actcggtcca attgggcaca atagtaatca gcagtgatag ttttattagt
     3961 tggcaacaat tcccagtgca cgggtccttg aacaccccac cagacacaga tcataatctt
     4021 ttttgggtga agatcaggct ttggcgtcgg tattcctttc tcaccgatcg gaagccattg
     4081 acgttttctg gaatggttaa catagagcac ccacttctca tctccagtaa ccagattgtt
     4141 cagccaatcg aactttcgac gaaaagttag aagttgagta caaacgttga cccgagtgag
     4201 cttctgtgat gccgaaagtt catgaggcac ccaaattcca agttttgaag taaaaccaag
     4261 gcgacccaag tgacgtgcaa ttgtgctatg acaacactca agcttctctt ccatttcacg
     4321 aagactaaga cgaggctctt cctccaccag cttcactagg tcttcttcat ttaactctac
     4381 cggtcgccca gaacgttcta tttcttcgag actgaagtcg ccgttcttga acttttgaaa
     4441 ccaactcttt gctgtattat aagagagtgc tccctcaccc atcgcaccgc atatgtttcg
     4501 ttccgcttcc attgctgaat gaccaagacg aaattcataa agaagaagca atctaacgtc
     4561 tcgacgttca agtttcatga ttgtcatcgt ggcaaagtag aaatggatga aatgaatctt
     4621 ttctgaaagg ggacgacccg accttgacac gacctacgat gaaaaaattt taaaactttc
     4681 tagaagtttt ggaaaaatat ttgaaaaaaa gtacaaaaac ttatgggaca acctgatatt
     4741 taataaatat tgctccgacg gattttctgc ctacgctgga cagaaaattt tgaatttaac
     4801 aattaattaa tttgaaatgg aaaaataata attttttaaa cattaattgg gtcgacaacg
     4861 taaaaatagt gatttttatt gacatttttt attgcttttt caataaaaag atattggagg
     4921 tatggggaat cgaaccccag acttctcgca tgcaaagcga gcgctctacc cctgagctat
     4981 acccccggac gagtttattg gtttgcgggg cggcttcgaa tagagatgcc agagtgtgtt
     5041 cgatgtacag caaaaaagga tggaggaggt ggtttgggaa gaacaatgga gatgtgttca
     5101 caaaaaaagt acgagattac tcaaaattgt ataCTATTCA ATTACAAATT TTGTAGAACT
     5161 TGATTTACCA AAAGATGTAA ATGTCTCGAC TGCATGctgc catgtgagca aaaggccagc
     5221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5701 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//